Series: Ernestly measurement
Measurement of bovine leukemia proviral DNA
Although this is not known very widely, enzootic bovine leukosis which is a type of bovine leukosis caused by the bovine leukemia virus has been increasing in recent years. We examined if the DNA (proviral DNA) integrated by the virus can be measured using a mobile PCR device.
Butchered meat (beef) whole blood
Blood stored in a frozen state (-80 degrees Celsius ) was centrifuged and rinsed with PBS twice before being used as a sample.
|μL||Remarks (final concentration)|
|Enzyme: KAPA 3G Plant (2.5 U/μL)||0.6||1.6U/reaction|
|F-Primer (100μM) ※1||0.1||1000nM|
|R-Primer (100μM) ※1||0.1||1000nM|
|Probe (100μM) ※1||0.05||600nM|
|PCR grade water||6.2|
Reaction sample total 16μL
The test result clarified that bovine leukemia proviral DNA can be measured from whole blood stored in a frozen state via direct PCR.
*1 Changed from the following literature
Made by Nihon Gene Research Laboratories Inc.
Reference literature: Journal of Virological Methods 189 (2013) 258– 264 We sincerely thank Miyakonojo Meat Inspection Center in Miyazaki Prefecture that provided cooperation for measurements.