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Can Legionella bacteria be measured?

Series: Ernestly measurement

Can Legionella bacteria be measured?

This section describes the measurement of Legionella bacteria. Infection of Legionella bacteria sometimes becomes a problem in bathing facilities. Periodic inspection is required but it takes almost one week.
If it can be inspected quickly on-site, it may help to take countermeasures quickly.
We checked if it can be measured using a mobile PCR device.

Sample

Legionella pneumophila ATCC33152 cultured strains

Pretreatment

Extracted and refined at AQUAS Corporation

Reaction composition
μL Remarks (final concentration)
Enzyme: KAPA 3G Plant (2.5 U/μL) 0.6 1.5U/reaction
Buffer (×2) 7.5 x1
F-Primer 900nM
R-Primer 900nM
Probe 200nM
MgCl2(25mM) 1.2 2mM
Sample 1.5
PCR grade water Up to 15

Reaction sample total 16μL

Program
Process Temperature Time
Initial denaturation 95℃ 15sec
Annealing/extension 58℃ 30sec 50cycle
Denaturation 95℃ 3sec
Results

Legionella bacteria were measured to the concentration of 10 copies/uL.

*This data was provided by Go!Foton Corporation.