Preparation of PCR reagents (Master Mix)

To use the mobile PCR device, reagents for PCR need to be prepared.
Generally speaking, reagents for PCR are the mixture of following materials.

Items (1) and (2) within all are specific for detection of each target.
Please refer “Preparation of primer and probe” on a different page.

Items (3) to (5) are commonly called “Master Mix” and various types of materials are sold by reagent companies.

For item (6), molecular grade water*1 can be purchased from dealers.

Since this mobile PCR device performs a quick reactions by moving the liquid in the flow path, it is recommended to use PCR reagents (Master Mix) appropriate.
In selecting, it is important as following.

  • Fast in enzyme reaction.
  • Antibody modification for a hot start. (Chemical modification be available in part.)
  • 5’ to 3’ exonuclease activity. (available for e.g.TaqMan®*2 probe.)

For concrete enzymes ridden on measurement examples, please refer to that.

*1)
We use for example “Invitrogen™ UltraPure DNase/RNase-Free Distilled Water” provided by Thermo Fisher Scientific Inc.
*2)
TaqMan® is a registered trademark of Roche Molecular Systems, Inc.
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